abstract：:The regulation of gene transcription allows yeast cells to respond properly to changing environmental conditions. Several protein complexes take part in this process. They involve RNA polymerase complexes, chromatin remodeling complexes, mediators, general transcription factors and specific transcriptional regulators....

journal_title：Folia microbiologica

authors： Drobná E,Bialková A,Subík J

更新日期：2008-01-01 00:00:00

abstract：:The root nodules of Phaseolus mungo (a herbaceous leguminous pulse) contained a high amount of 3-indolylacetic acid (IAA). A tryptophan pool present in the nodule might play the role of precursor for IAA production. From the root nodule a Rhizobium sp. was isolated. The symbiont produced a large amount of IAA (142 mic...

journal_title：Folia microbiologica

authors： Ghosh S,Sengupta C,Maiti TK,Basu PS

更新日期：2008-01-01 00:00:00

abstract：:Biochemical properties of a termostable alkaline phosphatase obtained from the mycelium extract of A. caespitosus were described. The enzyme was purified 42-fold with 32% recovery by DEAE-cellulose and concanavalin A-Sepharose chromatography. The molar mass estimated by Sephacryl S-200 or by 7% SDS-PAGE was 138 kDa an...

journal_title：Folia microbiologica

authors： Guimarães LH,Júnior AB,Jorge JA,Terenzi HF,Polizeli ML

更新日期：2007-01-01 00:00:00

abstract：:Candidiases, infections caused by germination forms of the Candida fungus, represent a heterogeneous group of diseases from systemic infection, through mucocutaneous form, to vulvovaginal form. Although caused by one organism, each form is controlled by distinct host immune mechanisms. Phagocytosis by polymorphonuclea...

journal_title：Folia microbiologica

authors： Raska M,Bĕláková J,Krupka M,Weigl E

更新日期：2007-01-01 00:00:00

abstract：:Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponenti...

journal_title：Folia microbiologica

authors： Pospísl S,Halada P,Petrícek M,Sedmera P

更新日期：2007-01-01 00:00:00

abstract：:The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were dete...

journal_title：Folia microbiologica

authors： Mattana CM,Vega AE,Escobar F,Sabini L,Zanón S,Puig de Centorbia ON

更新日期：2007-01-01 00:00:00

abstract：:A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to d...

journal_title：Folia microbiologica

authors： Pohanka M,Skládal P

更新日期：2007-01-01 00:00:00

abstract：:Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton &...

journal_title：Folia microbiologica

authors： Vohník M,Fendrych M,Albrechtová J,Vosátka M

更新日期：2007-01-01 00:00:00

abstract：:Thirty-four thiosemicarbazones and S-alkyl thiosemicarbazones, and some of their Zn(II) and Pd(II) complexes were obtained and purified to investigate antimicrobial activity. MIC values of the compounds were determined by the disc diffusion method against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pse...

journal_title：Folia microbiologica

authors： Kizilcikli I,Kurt YD,Akkurt B,Genel AY,Birteksöz S,Otük G,Ulküseven B

更新日期：2007-01-01 00:00:00

abstract：:The structural gene coding for YompC has been identified in the genome of a pathogenic strain of Yersinia enterocolitica O:9, and was subsequently cloned and sequenced. Detailed alignment of the deduced amino acid sequence showed that YompC is a member of the OmpC porin family with the highest degree of homology to Kl...

journal_title：Folia microbiologica

authors： Brzostek K,Raczkowska A

更新日期：2007-01-01 00:00:00

abstract：:Superoxide dismutases, both cytosolic Cu, Zn-SOD encoded by SOD1 and mitochondrial Mn-SOD encoded by SOD2, serve Saccharomyces cerevisiae cells for defense against the superoxide radical but the phenotypes of sod1A and sod2delta mutant strains are different. Compared with the parent strain and the sod1delta mutant, th...

journal_title：Folia microbiologica

authors： Dziadkowiec D,Krasowska A,Liebner A,Sigler K

更新日期：2007-01-01 00:00:00

abstract：:Two classes of newly synthesized amphiphilic compounds, phenolic antioxidants ("phenolics") and N-oxides exert in vivo antioxidant effects on live S. cerevisiae cells. Both groups have low toxicity, phenolics being more toxic than N-oxides and compounds with a longer alkyl chain having higher toxicity than those with ...

journal_title：Folia microbiologica

authors： Krasowska A,Sigler K

更新日期：2007-01-01 00:00:00

abstract：:An extracellular acid phosphatase isolated from the culture of a wild strain Aspergillus niger, producing the dephosphorylating 3-phytase, was obtained in a homogeneous form by sequential application of ultrafiltration through PS 50 membrane, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE-Sep...

journal_title：Folia microbiologica

authors： Gargova S,Sariyska M,Angelov A,Stoilova I

更新日期：2006-01-01 00:00:00

abstract：:The screening of 17 SAg genes of S. aureus isolated from the sputum of cystic fibrosis (CF) patients revealed that among 47 genetically different strains, 39 (83 %) carried SAg genes. Superantigens forming enterotoxin gene cluster were detected in 20 strains. The 2nd most common superantigen type was selk detected in ...

journal_title：Folia microbiologica

authors： Bania J,Dabrowska A,Rózalska B,Sadowska B,Wieckowska-Szakiel M,Korzekwa K,Zarczyńska A,Bystroń J,Chrzanowska J,Molenda J

更新日期：2006-01-01 00:00:00

abstract：:The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSP) as well as of succinate dehydrogenase (SDG), NADH dehydrogenase (NDG) and fumarate hydratase (FHT) were examined in relation to mitochondrial ultrastructure changes in Aspergillus niger exposed to N,N-bis(3-aminopropyl)dodecy...

journal_title：Folia microbiologica

authors： Kuźniak E,Wyrwicka A,Gabara B,Koziróg A,Skłodowska M

更新日期：2006-01-01 00:00:00

abstract：:The characterization of 11- and 18-residue peptaibols (peptides synthesized by peptide synthetases) at Trichoderma harzianum CECT 2413 (a filamentous fungus) was performed. Using a heterologous probe from tex1, the only peptaibol synthetase cloned and characterized so far in Trichoderma species, was cloned; a region t...

journal_title：Folia microbiologica

authors： Vizcaíno JA,Cardoza RE,Dubost L,Bodo B,Gutiérrez S,Monte E

更新日期：2006-01-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on viral and host cellular activities during infection, including induction of the cell cycle G2 arrest, and cell death in both human cells and the fission yeast Schizosaccharomyces pombe. We show that treament of exponential-pha...

journal_title：Folia microbiologica

authors： Antal J,Pesti M

更新日期：2006-01-01 00:00:00

abstract：:Twenty-one healthy bottle-fed infants were screened monthly (1-4 months) for bifidobacteria in their stools. Bifidobacteria were detected by culture and isolates specified by PCR. Alternatively, direct PCR in undiluted fecal suspensions was carried out for detection of bifidobacteria under the cultural detection level...

journal_title：Folia microbiologica

authors： Mullié C,Romond MB,Izard D

更新日期：2006-01-01 00:00:00

abstract：:Killer strains of Saccharomyces cerevisiae producing killer toxin K1 kill sensitive cells but are resistant to their own toxin. It is assumed that in the producer, an effective interaction between the external toxin and its plasma membrane receptor or the final effector is not possible on the grounds of a conformation...

journal_title：Folia microbiologica

authors： Valis K,Masek T,Novotná D,Pospísek M,Janderová B

更新日期：2006-01-01 00:00:00

abstract：:The sorption ability of Candida utilis biomass for cadmium ions with accumulating competence of dried cells and cells in alginate was compared. After an optimization of process conditions (pH 5.5, biomass concentration 1 g/L and c0 50 mg/L), the cadmium sorption capacity of dried yeast biomass was perceptibly higher t...

journal_title：Folia microbiologica

authors： Kujan P,Prell A,Safár H,Sobotka M,Rezanka T,Holler P

更新日期：2006-01-01 00:00:00

abstract：:Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (...

journal_title：Folia microbiologica

authors： Piknová M,Bíres O,Javorský P,Pristas P

更新日期：2006-01-01 00:00:00

abstract：:The color of light (white, red, blue, and green) had a significant effect on the growth and reproductive processes (both in the nucleocytoplasmic and chloroplast compartment of the cells) in synchronous cultures of Scenedesmus obliquus. This effect decreased in the order red > white > blue > green. In the same order, ...

journal_title：Folia microbiologica

authors： Cepák V,Pribyl P,Vítová M

更新日期：2006-01-01 00:00:00

abstract：:Bacillus subtilis strains expressing listeriolysin O (LLO) and simultaneously LLO and p60 protein were constructed. The effect of p60 protein on hemolytic activity and on the invasion of professional phagocytes was demonstrated in the absence of other virulence factors of L. monocytogenes. The hemolytic activity of LL...

journal_title：Folia microbiologica

authors： Krawczyk-Balska A,Markiewicz Z,Bielecki J

更新日期：2005-01-01 00:00:00

abstract：:Verotoxin-producing Escherichia coli (VTEC) are nowadays among the most important emerging group of food-borne pathogens (VTEC strains cause gastroenteritis that can be complicated by the hemorrhagic colitis or hemolytic uremic syndrome, HUS). Escherichia coli 026 producing verotoxin 2 was isolated and its identity co...

journal_title：Folia microbiologica

authors： Liptáková A,Siegfried L,Kmetová M,Birosová E,Kotulová D,Bencátová A,Kosecká M,Bánovcin P

更新日期：2005-01-01 00:00:00

abstract：:A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for the acmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic i...

journal_title：Folia microbiologica

authors： Prodelalová J,Spanová A,Rittich B

更新日期：2005-01-01 00:00:00

abstract：:Euglena ignobilis cells in natural puddle water of pH 7.8, when kept at 21 +/- 2 degrees C and under continuous light (intensity of approximately 30 micromol m(-2) s(-1)) in a culture chamber, decreased their speed of movement from > or = 78000 microm/min (after a 12-h cultivation), to 850-1300 microm/min after 18 h. ...

journal_title：Folia microbiologica

authors： Gupta S,Agrawal SC

更新日期：2005-01-01 00:00:00

abstract：:A total of 20 putative strains of Erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. Fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody f...

journal_title：Folia microbiologica

authors： Kokosková B,Mráz I

更新日期：2005-01-01 00:00:00

abstract：:Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans s...

journal_title：Folia microbiologica

authors： Kalkanci A,Bozdayi G,Biri A,Kustimur S

更新日期：2005-01-01 00:00:00

abstract：:The plasma membrane and mitochondria of bottom fermenting brewer's yeast obtained as a by-product of industrial beer production were isolated and the lipid fraction was analyzed. The phospholipid content accounted for 78 mg/g protein in the plasma membrane and 59 mg/g protein in the mitochondria. Major phospholipids i...

journal_title：Folia microbiologica

authors： Blagović B,Rupcić J,Mesarić M,Marić V

更新日期：2005-01-01 00:00:00

abstract：:A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating age...

journal_title：Folia microbiologica

authors： Kuhad RC,Kapoor RK,Lal R

更新日期：2004-01-01 00:00:00

abstract：:Microorganisms in the midgut and rectum of the honeybee were enumerated and characterized. Counts of aerobic microorganisms were distinctly lower than counts of anaerobes (10(5)-10(6) viable cells per g of intestinal content vs. 10(8)-10(9) per g). Total numbers of anaerobic microorganisms were almost identical with t...

journal_title：Folia microbiologica

authors： Kacániová M,Chlebo R,Kopernický M,Trakovická A

更新日期：2004-01-01 00:00:00

abstract：:Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child w...

journal_title：Folia microbiologica

authors： Vlková E,Rada V,Bujnáková D,Kmet V

更新日期：2004-01-01 00:00:00

abstract：:Genome projects produce a huge amount of sequence information. As a result, the focus of genomics research is turning toward deduction of functional information about newly discovered genes. Thus structural genomics paves the way for a new discipline called functional genomics by providing the information required for...

journal_title：Folia microbiologica

authors： Majtán T,Bukovská G,Timko J

更新日期：2004-01-01 00:00:00

abstract：:A group of sixteen esculin-positive fluorescent pseudomonads isolated from an underground brook flowing through a cave complex was characterized by biotyping, multiple enzyme restriction fragment length polymorphism analysis of 16S rDNA (MERFLP), ribotyping and whole-cell fatty-acid methyl-esters analysis (FAME). All ...

journal_title：Folia microbiologica

authors： Svec P,Stegnerová H,Durnová E,Sedlácek I

更新日期：2004-01-01 00:00:00

abstract：:A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were purified to homogeneity. Their molar mass, estimated by SDS-PAGE, were 21 and 24 kDa, respectively. Both enzymes are glycoproteins with 50 degrees C temperature optimum; optimum pH was 6.0-6.5 for ...

journal_title：Folia microbiologica

authors： Fialho MB,Carmona EC

更新日期：2004-01-01 00:00:00

abstract：:Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacilli...

journal_title：Folia microbiologica

authors： Cernohorská L,Votava M

更新日期：2004-01-01 00:00:00

abstract：:A wild-type strain of Candida albicans (S1, ATCC 10261) was used to obtain stable auxotrophic colony morphological mutants (mutant M5 producing only true hyphae and mutant M2 containing 90 % blastospores and 10 % pseudohyphae) by induced mutagenesis. A hybrid was produced by somatic hybridization between these 2 mutan...

journal_title：Folia microbiologica

authors： Novák A,Vágvölgyi C,Emody L,Pesti M

更新日期：2004-01-01 00:00:00

abstract：:We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial po...

journal_title：Folia microbiologica

authors： Moragues M,Comas-Riu J,Vives-Rego J

更新日期：2004-01-01 00:00:00

abstract：:Frequency of heterocytes and nitrogenase activity (NA) under light and dark cultivation conditions was determined in 12 cyanobacterial strains isolated from various soil habitats. In spite of a high variability, significant differences in NA among the strains were found in response of light and dark cultivation. Relat...

journal_title：Folia microbiologica

authors： Hrouzek P,Lukesová A,Simek M

更新日期：2004-01-01 00:00:00

abstract：:Sodium fluoride showed pH-dependent physiological responses in the two test microalgae Anabaena khannae and Chlorococcum humicola. A. khannae showed severe membrane damage with fluoride at low pH with leakage of pigments and electrolytes. Annihilation of photosynthesis along with inhibition in 14C uptake was observed ...

journal_title：Folia microbiologica

authors： Bhatnagar M,Bhatnagar A

更新日期：2004-01-01 00:00:00